Moe Mahjoub, Tim Stearns (2010) CIL:10061, Mus musculus, tracheal epithelial cell. CIL. Dataset

Mouse tracheal epithelia cells (MTEC) were grown at air-liquid interface to induce differentiation, centriole amplification and ciliogenesis. Cells were fixed with ice-cold methanol for 10 min. Samples were stained with antibodies against acetylated tubulin to mark cilia (red), the centriolar protein Cep120 to mark basal bodies (green), and anti-ZO-1 to mark the cell-cell junctions (light blue). Secondary antibodies were Alexa 594 for acetylated tubulin, Alexa 488 for Cep120, and Cy5 for Zo-1. Nuclei were stained using DAPI (blue). Images were captured using Openlab 4.0.4 software controlling an Axiovert 200M microscope (Carl Zeiss, Inc.) with a 100X 1.4 NA objective.

小鼠气管上皮细胞（MTEC）在气-液界面培养，以诱导分化、中心粒扩增和纤毛生成。细胞经冰冷的甲醇固定10分钟。样本经抗乙酰化微管蛋白抗体染色以标记纤毛（红色），抗Cep120蛋白抗体染色以标记基座体（绿色），以及抗ZO-1抗体染色以标记细胞间连接（浅蓝色）。使用Alexa 594标记乙酰化微管蛋白，Alexa 488标记Cep120，Cy5标记Zo-1作为二抗。细胞核使用DAPI（蓝色）染色。图像采用Openlab 4.0.4软件，通过控制Axiovert 200M显微镜（卡尔蔡司公司）的100X 1.4 NA物镜进行捕获。