WWOX gene and estrogen receptor interplay in breast cancer

The aim of presented study was to assess the relationship between the presence of estrogen receptor and WWOX gene in breast cancer cell lines. Overall design: The study was based on estrogen-responsive (MCF7) and estrogen-unresponsive (MDA-MB-231, BT20) cell lines, for which depending on native expression, WWOX gene was either induced by retroviral transfection (MDA-MB-231) or silenced with shRNA (MCF7, BT20), and changes in the phenotype were estrogen stimulated. We have employed nAnT-iCAGE methodology for high-throughput gene expression profiling.