Data for: OsDSSR1, a novel small peptide, enhances drought tolerance in transgenic rice

RNA-seq analysis was carried out using transgenic lines OX37. Fourteen-day-old rice seedlings were treated with or without 20% solutions of PEG6000 for 6h. Total RNA was isolated with Trizol reagent (Invitrogen, USA) from seedlings of transgenic and 93-11 plants grown under normal or PEG conditions. Material from 10 plants of each genotype was pooled for RNA extraction. The concentration and quality of each RNA sample were determined using a NanoDrop 2000TM micro-volume spectrophotometer (Thermo Scientific, Waltham, MA, USA) and gel electrophoresis. The rice RNA samples were sent to BGI (The Beijing Genomics Institute at Shenzhen, China) for sequencing.本研究采用转基因株系OX37开展RNA测序（RNA-seq）分析。选取14日龄水稻幼苗，分别施加20% PEG6000溶液处理与空白对照，处理时长为6小时。从正常培养及PEG处理条件下培养的转基因株系与93-11植株的幼苗中，采用Trizol试剂（Invitrogen，美国）提取总RNA；每个基因型选取10株植株的材料混合后用于RNA提取。使用NanoDrop 2000™超微量分光光度计（赛默飞世尔科技，美国马萨诸塞州沃尔瑟姆）与凝胶电泳检测各RNA样品的浓度与质量。最终将水稻RNA样品送至BGI（中国深圳北京基因组研究所）进行测序。