Fibroblast orchestration of inflammaging via NF-kB activation (Aged vs Young Lung Fibroblasts and Epithelium)

In this study we used scRNA sequencing to compare lung fibroblast and epithelial populations from young and aged mice. We identified enrichment of a NF-kB activation signature in aged relative to young fibroblasts, particularly in the adventitial population. Overall design: Cells were sorted from one 2 month and one 22 month old wildtype (C57BL/6) mouse. Fibroblasts were identified as lineage negative (CD45-Epcam-CD31-) cells. Epithelial cells were identified as CD45-CD31-Epcam+. After sorting these two cell populations were mixed to generate a 70% fibroblast/30% epithelial cell sample for sequencing.