Comparison of transient transformation efficiencies in different plant materials by using agroinfiltration.

Note: three samples per material were investigated for ten 2 mm2 areas of agroinfiltrated epidermal cells. Each efficiency value represents the mean of transformation efficiencies of thirty replicates of 2 mm2 epidermal areas from three samples (the percentage of positive cells in total cells per unit area), and the standard errors were calculated by using Excel. Different capital and lowercase letters within the same column exhibit significant difference at the 1% and 5% probability level according to the Duncan test of SPSS 10.0 statistic analysis. For onion two-day pretreatment was conducted before agroinfiltration.