Influence of Crude Phenolic Extract from Coffee Pulp Supplementation in Broiler Diets on Productive Performance, Gut Microbiota, and Immune Response

Our study hypothesized that crude phenolic extract (CE) from coffee pulp could enhance microbial homeostasis, gut health, and immune response in broilers, ultimately improving their growth performance. The research aimed to assess the antimicrobial properties of CE in vitro and evaluate its dietary effects on broiler productivity and health. To test this, we conducted in vitro assays to determine CE’s antibacterial and anti-adherent properties. Additionally, 160 one-day-old Ross 308 male broilers were randomly assigned to four dietary treatments: a control group (CE0) and groups supplemented with 0.25 g/kg (CE0.25), 0.5 g/kg (CE0.5), and 1 g/kg (CE1) of CE. Growth performance, gut microbiota composition, and immune markers were measured throughout the trial. Further research should focus on long-term effects and potential applications in commercial poultry production.本研究提出假说：咖啡果肉来源的粗酚提取物（crude phenolic extract, CE）可改善肉用仔鸡的微生物稳态、肠道健康与免疫应答，最终提升其生长性能。本研究旨在体外评估CE的抗菌特性，并探究其作为日粮添加剂对肉用仔鸡生产性能与健康状况的影响。 为验证该假说，本研究开展体外试验以测定CE的抗菌与抗黏附特性。此外，本研究选取160只1日龄罗斯308（Ross 308）雄性肉用仔鸡，随机分为4个日粮处理组：对照组（CE0）以及分别添加0.25 g/kg（CE0.25）、0.5 g/kg（CE0.5）和1 g/kg（CE1）CE的试验组。试验全程测定肉用仔鸡的生长性能、肠道菌群组成与免疫标志物水平。 未来研究应聚焦于CE的长期应用效果及其在商品肉鸡养殖生产中的潜在应用价值。