ADAR1 as a Key Regulatory Molecule between MiR-432 and Circ_0000418 in Antidepressant Action

In this study, all the data were analyzed by using GraphPad Prism 7.0 and IBM SPSS Statistics 23.0. All the data were expressed as the means±SD. The behavior data of C1 group and CUS1 group was analyzed by u test. In addition, t test was used to analyze the variance for the groups with and without chronic unpredictable stress, the groups with and without ADAR1 target intervention in the mice, as well as the groups with and without transfection in SH-SY5Y cell line. Two-way ANOVA was used to determine whether there is an interaction between chronic unpredictable stress and ADAR1 target intervention (two independent variables) on ADAR1 mRNA expression, BDNF mRNA expression, circ_0000418 expression, and miR-432 expression (dependent variable) respectively among the mice. One-way ANOVA was used for comparison of samples among multiple groups. Tukey’s test was used for significance analysis. p< 0.05 was considered to be statistical significantly.本研究所有数据均采用GraphPad Prism 7.0与IBM SPSS Statistics 23.0进行统计分析。所有数据以均值±标准差（mean±SD）形式呈现。C1组与CUS1组的行为学数据采用u检验进行分析。此外，针对伴有与不伴有慢性不可预见性应激（chronic unpredictable stress, CUS）的小鼠组、接受与未接受ADAR1靶点干预（ADAR1 target intervention）的小鼠组，以及转染与未转染SH-SY5Y细胞系（SH-SY5Y cell line）的组别，均采用t检验进行方差分析。双向方差分析（two-way ANOVA）用于分别探究慢性不可预见性应激与ADAR1靶点干预这两个自变量，在小鼠ADAR1 mRNA表达、BDNF mRNA表达、circ_0000418表达以及miR-432表达这几项因变量上是否存在交互作用。单因素方差分析（one-way ANOVA）用于多组样本间的比较，Tukey检验（Tukey’s test）用于显著性分析。当p<0.05时，认定差异具有统计学意义。