Splicing characteristics of different human reference annotations.

Coding transcripts from 3 reference annotations of the human genome��namely, Gencode, RefSeq, and EnsEmbl��have been evaluated for their splicing properties. For each reference annotation, the number of loci obtained by our clustering method in comparison to the number of transcripts is given. Subsequently we present the total number of variations in the exon�Cintron structure detected by applying the AStalavista method and the subset of them that forms AS events according to Definition 4 with canonical splice sites. Finally, the computation time on a standard desktop PC is reported.