Transcriptome analysis of LRP5- and LRP6-depleted HCC38 cells.

In order to characterize the differences between the co-receptors LRP5 and LRP6, we have analyzed the transcriptome of HCC38 cells - a triple negative breast cancer cell line - 24, 48 and 72 hours following the depletion of LRP5 or LRP6 using siRNAs. Cells were transfected with control siRNA, 2 different siRNA targeting LRP5 (#2, #4), or 2 different siRNA targeting LRP6 (#7, #8).Total RNA was extracted and hybridized onto Gene 2.1 Affymetrix chips. Duplicates for each condition were included in the experiment.