Massively parallel sequencing is rapidly emerging as an efficient way to quantify biodiversity at all levels, from genetic variation and expression to ecological community assemblage. However, the num
(a) Accession number of genes found in Fragaria vesca genome (http://www.strawberrygenome.org/andhttp://www.rosaceae.org/) that may be amplified by the designed primer pairs. (b) Theoretical melting t
Amplicons for NGS were prepared following cloning of PCR fragments into mammalian expression vectors as outlined in Fig 8A. The average number of full-length reads was determined based on the percenta