16S sequenceing date for cecal microbiota of pullets under acute heat stress

Heat stress in poultry is deleterious to productive performance. Many studies have reported that chlorogenic acid (CGA) has positive effects in attenuating heat stress. This study examined the effects of dietary supplementation with CGA on the intestinal health and cecal microbiota composition of young hens challenged with acute heat stress. 100-day-old Hy-line brown pullets were randomly divided into four groups. The control group (C) and heat stress group (T1) received a basal diet. T2 group and T3 group received a basal diet supplemented with 300 mg/kg and 600 mg/kg CGA respectively, for two weeks before acute heat stress exposure. Pullets of T1, T2, and T3 group were exposed to 38℃ for 4 h while the control group was maintained at 25℃. 16 S rDNA Gene Sequencing for Cecal Microbiome was performed an Illumina Miseq.家禽热应激对其生产性能具有有害影响。多项研究已证实，绿原酸（chlorogenic acid, CGA）在缓解热应激方面具备积极功效。本研究探究了日粮添加绿原酸对急性热应激造模青年母鸡肠道健康与盲肠微生物群组成的影响。选取100日龄海兰褐（Hy-line brown）育成母鸡，随机分为4组：对照组（C组）与热应激组（T1组）饲喂基础日粮；T2组与T3组则在急性热应激暴露前两周，饲喂分别添加300 mg/kg、600 mg/kg绿原酸的基础日粮。T1、T2、T3组育成母鸡置于38℃环境中暴露4小时，对照组则维持于25℃环境。本研究采用Illumina Miseq平台开展盲肠微生物组的16S rDNA基因测序分析。