Air Liquid Interface Model of Gastric Mucosa and H.pylori Infection. Homo sapiens

The Air Liquid Interface Model of Gastric Mucosa allows to provide a fully polarized epithelium accessible to infection experiments with H.pylori. The influence of several factors on immune response has been studied. Overall design: Primary cells were isolated from healthy tissue of surgery specimen taken from patients undergoing sleeve resections, seeded on collagen coated filters and cultured after 3d in air liquid interface. Total RNA was extracted from samples and hybridized on Agilent-8x60k Human custom arrays as single-color hybridizations.