Analysis of the antifungal potential of <em>Macrocybe titans</em> extract against <em>Candida albicans</em> - Supplementary material

Aim: To investigate the antifungal potential of <em>Macrocybe titans</em> extracts against <em>Candida albicans</em>. Material &amp; methods: Extracts were obtained as aqueous (EfraMat-22 and EfraMat-45) and methanolic/ethyl acetate fractions. Results: Broth microdilution and disk diffusion assays showed that EfraMat-45 provided the best results in terms of minimum inhibitory concentration. Scanning electron microscopy analysis revealed morphological changes and slight damage on the surfaces of cells exposed to EfraMat-45 at the MIC. Fluorescence microscopy analysis of the yeasts showed cell elongation. EfraMat-45 presented high levels of phenolic compounds and flavonoids, high antioxidant activity and absence of <em>in vitro</em> cytotoxicity. Conclusion: The results indicated that the aqueous extract of <em>M. titans</em> is highly promising as an antifungal agent.