This file provides further information on materials and methods used in this study, as well as one supplemental table, and images of western blot used in the figure
aEvaluated by densitometry of PAGE Gels Stained with Coomassie Blue R-250 bPurified according to cGMP standards ND = not done WT = wild type CH = codon harmonized
A. Protein lysates were harvested from fibroblasts infected with TB40/E wild type (WT), UL88-deficient, or UL88Rev HCMV at MOI of 3 at 96hpi. Blots were probed for viral proteins UL88, pp28, and tubul
Densitometric quantification of protein expression from Western Blot using ImageJ analysis.Cell lines used: NMR Lung Fibroblast (NLF), NMR Skin Fibroblast (NSF), Mouse Skin Fibroblast (MSF) and NIH3T3