ATAC seq of cardiomyocyte nuclei of H2BmCh iOE mouse

Cardiomyocyte nuclei are constantly exposed to mechanical stress. It remains mysterious how they maintain nuclear shape throughout a life. We found that high-level overexpression of H2B-mCherry in mouse cardiomyocytes causes elongation of nuclei with disrupted spatial organizations of heterochromatin at microscopic level. Cardiomyocyte nuclei in H2B-mCherry iOE mice show heterochromatin disruption at 2 weeks after TMX induction, leading to nuclear deformation, resulting in fatal heart failure at 6 weeks after TMX induction. The purpose of this ATAC seq is to investigate the change of open-close chromatin structures accompanied with heterochromatin disruption. We isolated cardiomyocyte nuclei from H2B-mCherry iOE mouse hearts at 0 days and 2 weeks post-TMX with FACS, subjected to library construction for ATAC seq.