Whole-genome sequencing of chromatin in Hacat cells overexpressing WT1

We report the application of chip sequencing in the search for target genes of transcription factor .Chip-seq efficiently detect genome-wide segments of DNA interacting with transcription factors.We overexpressed the transcription factor WT1 in hacat cells, and then performed chip experiments to collect the DNA fragments bound to WT1, which were then sequenced.Finally, we proved that interleukinin is the target gene of WT1, providing a new target for clinical research and treatment of skin diseases. Hacat cells overexpressing WT1 plasmid were sequenced and blank plasmid sets were established.