A LINE-1 retrotransposon promotes SEMA3C expression as a cis-regulatory enhancer to sustain breast cancer stem cell survival

Transposable elements (TEs), which are under tight epigenetic control, have been co-opted as cis-regulatory elements to regulate gene expression in development and cancer. Among them, long interspersed element 1 (LINE1) retrotransposons are the most abundant and exhibit high activity in embryonic stem cells.To investigate the precise roles of LINE1 in breast cancer stem cells (BCSCs) , we performed total RNA-seq analysis of non-CSCs and CSCs enriched from EO771 cells. Overall design: Comparative gene expression profiling analysis of RNA-seq data for non-CSCs and CSCs.