RNA-Seq of human AML samples, and human cells transformed with MLL-ENL or MLL-AF6

RNA-Seq of human AML samples, and human cord blood cell lines transformed with MLL-ENL or MLL-AF6. Cells extracted from MLL-fusion AML patients are bulk, cord blood cells have been sorted to HSC, LMPP, CMP and GMP. Cells are either normal, or transformed with MLL-AF6 or MLL-ENL. The cell lines have been grown for a short time (ST) or long time (LT). Only the long time is an established pure cell line, short time is a mixture of MLL-transformed and untransformed cells. Several cords were used for the sorting (CB1, CB2, CB3, 135, 141...) and these represent biological replicates. Several samples were sequenced several times in different lanes and results were merged together for the analysis (rep1,rep2...). Samples were used to determine the different effect of MLL-fusions in different cell types just after the transformation, and after a longer time period. The cord blood samples were used as references to predict which of the patients originated from which cell-type of origin.