Genome wide examination of alterations in mRNA translation in striatal D1 neurons in Fmr1 model mice

We immunoprecipitated GFP tagged ribosomes from Drd1 receptor-expressing cells in the striatum from adult WT and fragile X syndrome model mice and sequenced the co-purifying mRNA (IP). We also sequenced the RNA in the striatal lysate (total). Examination of ribosome bound mRNA in D1 neurons from FMRP mutant mice.我们从成年野生型（WT）及脆性X综合征（fragile X syndrome）模型小鼠的纹状体中，对表达多巴胺D1受体（Drd1）的细胞内的GFP标记核糖体进行免疫沉淀（immunoprecipitation），并对共纯化得到的mRNA进行测序（免疫沉淀组，IP）。同时，我们还对纹状体裂解液中的RNA进行测序（总组，total）。本数据集旨在检测FMRP（脆性X智力低下蛋白，Fragile X Mental Retardation Protein）突变小鼠D1神经元内结合于核糖体的mRNA。