HPV genome expression at invasive cervical carcinomas infected by multiple HPV genotypes

Multiple HPV genotypes infection is frequently detected in HPV+ cervical lesions, however it is not well stablished how is the different viral interaction during the carcinogenic process. Here we carried out a comprehensive study to characterize the multiple HPV genome expression and integration by RNA-Seq analysis in 19 invasive cervical carcinomas with HPV coinfections. Analysis of tumoral DNA by a hybridization kit indicated multi-infection ranging from 2 to 6 different HPV genotypes, without a preferential species coinfection. The expression analysis showed that a single HPV genotype preferentially expressed its genome, might indicating a competition between the infecting virus. Finally, the search for HPV/human chimeric transcripts indicated integration from just one HPV in almost all samples, corroborating the expression findings. Overall design: RNA-Seq analysis of 19 uterine cervix tumor with multiple HPV genotype infection. Please note that chimeric transcripts were not identified in HPVco_2, HPVco_6, HPVco_10, HPVco_13, and HPVco_15-17 samples and thus no fasta data file is provided.