Quantitative analysis of the splice variants expressed by the major hepatitis B virus genotypes

RNA-seq of Huh7 cells transfected with replication competent clones of HBV genotypes A2, B2, C2, and D3. Overall design: Total RNA samples were purified using RNeasy kit (Qiagen, Hilden, Germany). The libraries were prepared using TruSeq stranded Total RNA sample preparation kit with Ribo-Zero according to the manufacturer's protocol and sequenced using HiSeq 2500 (Illumina, CA, USA), generating 125-bp paired-end reads.