Miguel Vicente-Manzanares, Karen Newell-Litwa, Alexia I. Bachir, Leanna A. Whitmore, Alan Rick Horwitz (2011) CIL:25846, Cricetulus griseus, epithelial cell. CIL. Dataset

Time-lapse of a CHO.K1 cell cotransfected with very low levels of GFP-MIIA (myosin IIA, green) and paxillin (magenta). Yellow arrowheads mark paxillin-containing adhesions that disassemble concomitantly to their MIIA-decorated bundle (marked with arrows) as the movie progresses. Total internal reflection fluorescence (TIRF) images were acquired on an inverted microscope (IX70; Olympus) with a 1.45 NA (oil) Plan-Apochromat 60× TIRFM objective lens. Images were acquired with a charge-coupled device (CCD) camera (Retiga Exi; Qimaging). Video corresponds to Fig 5C and Video 5 from J Cell Biol 2011 193:381-396.

该数据集展示了一种CHO.K1细胞在低水平共转染绿色荧光蛋白标记的肌动蛋白IIA（MIIA，绿色）和品红色标记的paxillin（品红色）的延时摄影。黄色箭头标记了含有paxillin的粘附连接，随着影片的推进，这些粘附连接与其MIIA标记的束（用箭头标记）同步解聚。总内反射荧光（TIRF）图像是在倒置显微镜（IX70；Olympus）上使用1.45 NA（油）Plan-Apochromat 60× TIRFM物镜采集的。图像使用电荷耦合器件（CCD）相机（Retiga Exi；Qimaging）采集。视频对应于J Cell Biol 2011年第193卷第381-396页中的图5C和视频5。