The effect of PROTAC-mediated depletion of TAF1 on gene expression

The goal of this experiment was to determine the change in gene expression after transient depletion of the TAF1 transcription factor (aka TFII-250) using a PROTAC molecule (ZS3-046) containing GNE371 as TAF1 ligand and pomalidomide as CRBN ligand. Overall design: Molm13 cells were treated with 100 nM ZS3-046 for 8 hr in triplicate. Total RNA samples were prepared using RNeasy min kit (Qiagen). RNA-seq analysis including rRNA depletion, library preparation, multiplexing and cluster generation, sequencing on Illumina HiSeq2500, and differential gene expression initial analysis, were performed by Azenta Life Sciences.