Here, we present a large RNA-Seq dataset of Human alphaherpesvirus 1, derived from long-read sequencing. Pooled cDNA libraries were sequenced using the Pacific Biosciences Sequel platform and the Oxford Nanopore’s MinION device. CDNA prepered from individual time points post-infection and pooled poly(A)-selected mRNA was sequenced using the MinION sequencer.. Long-read transcriptomic dataset of Human alphaherpesvirus 1.

Here, we present a large RNA-Seq dataset of Human alphaherpesvirus 1, derived from long-read sequencing. Pacific Biosciences Sequel platform and a Oxford Nanopore’s MinION device with direct RNA sequencing and cDNA sequencing methods were used. PolyA or CAP selected or ribodepleted mRNAs were isolated from different time points after viral infection and reverse transcribed into cDNA using oligo(d)T20 or random primers.