Micro RNA in follicular fluid collected from young and aged cows

Follicular fluid (FF) is a sole environment for oocyte growth and contains myriad of molecules to support oocyte growth. Aging reduces oocyte quality which is a causal factor for aged associated infertility. And an ability of the follicular fluid (FF) to support oocyte maturation declimes as cows aged. Present study addresses the effect of aging on frequency of miRNAs in the FFs collected from young and aged cows. This data is smallRNAseq of the exosome extracted from FFs of antral follicles (3-5 mm in diameter) of young (25-50 months in age) and aged (over 140 months in age) Japanese Black Cows. Ovaries were collected at a slaughterhouse and transported to the laboratory within 4 hours. And then FF were aspirated from antral follicles. Exosome RNA was extracted from exosomes using a SeraMir Exosome RNA Amplification Kit (System Biosciences). RNA quality and concentration were examined using a Bioanalyzer (Agilent technologies, Palo Alto, CA, USA); a cDNA library was constructed using an Illumina TruSeq Small RNA Library Preparation Kit (Illumina, San Diego, CA, USA) for RNA from FFs. Clusters were generated on a cBot (Illumina), and one lane of the multiplied samples was sequenced as 75 bp reads (single read) on the NextSeq (Illumina). Image analysis, base calling and quality filtering were performed using the bcl2fastq2 v2.18.0.12 (Illumina) following the manufacturer's instructions. Sequence data were filtered to discard the adapter sequence, ambiguous nucleotides and low-quality sequences. The remaining sequence data were aligned to the Bos Taurus genome sequence (ARS-UCD1.2/bosTau9) to count sequence reads.