Mechanistic insights into the role of the canonical poly(A) polymerase Pla1 in RNA surveillance by the fission yeast MTREC complex. Mechanistic insights into the role of the canonical poly(A) polymerase Pla1 in RNA surveillance by the fission yeast MTREC complex

In this study we analysed the functional relevance of the interaction between Pla1 and the MTREC complex core component Red1 using various high throughput sequencing analyses performed on different Pla1-Red1 interaction mutants.Our analyses revealed that as part of MTREC complex, Pla1 hyper-adenylates Cryptic Unstable Transcripts (CUTs) for their efficient degradation. Interestingly, our H3K9(me)2 ChIP seq. data analysis also showed us that a functional Red1-Pla1 interaction was also required for the efficient assembly of the fission yeast facultative heterochromatic islands. Together, our data suggest a complex interplay between the RNA surveillance and 3’-end processing machineries. Overall design: Detailed analysis of two Pla1-Red1 interaction mutants namely red1W298A/F305A and red1△288-345 with WT and total red1△ control strains using high throughput poly(A)+ RNA sequencing, total RNA sequencing, RNA-immunoprecipitation (RIP) followed direct RNA sequencing and ChIP seq. analysis.