Vocal and tongue exercise in early to mid-stage Parkinson disease using the Pink1-/- rat

Vocal and swallowing deficits are common in Parkinson disease (PD). Because these impairments are resistant to dopamine replacement therapies, vocal and lingual exercise are the primary treatment, but not all individuals respond to exercise and neural mechanisms of treatment response are unclear. To explore putative mechanisms, we used the progressive Pink1-/- rat model of early to mid-stage PD and employed vocal and lingual exercises at 6- and 10-months of age in male Pink1-/- and wild type (WT) rats. We hypothesized that vocal and lingual exercise would improve vocal and tongue use dynamics and increase serotonin (5HT) immunoreactivity in related brainstem nuclei. Rats were tested at baseline and after 8 weeks of exercise or sham exercise. At early-stage PD (6 months), vocal exercise resulted in increased call complexity, but did not change intensity, while at mid-stage (10 months), vocal exercise no longer influenced vocalization complexity. Lingual exercise increased tongue force ge...,
Experimental design

All procedures were approved by the University of Wisconsin School of Medicine and Public Health Institutional Animal Care and Use Committee (IACUC; protocol M005177) and were conducted in accordance with the NIH Guide for the Care and Use of Laboratory Animals (National Research Council Committee). The following experimental design was performed with two separate cohorts of male Long-Evans rats, aged 6 months and 10 months at the time of final testing, respectively. In each cohort, three experimental groups were compared: Pink1-/- rats were randomized to receive vocal and lingual exercise (group 1) or sham exercise (group 2), and WT rats all received sham exercise as a control (group 3) (Table 1) (Broadfoot et al., 2023). Vocal and tongue exercise, and sham vocal and tongue exercise were completed for 8 weeks prior to time of final testing (baseline 4 months, and baseline 8 months for each age group, respectively), following standard vocal and lingual exercise pro..., , # Vocal and Tongue Exercise in Early to Mid-stage Parkinson Disease using the Pink1-/- Rat: Open Source Data

 **Description of the data, software, and file structure** 

 We have uploaded the relative optical density averages for the 6month old animals (*6mo_RoD_Averages_Master_Sheet_IDs_Removed.xlsx*), the relative optical density averages for the 10 month old animals (*10mo_RoD_Averages_Master_Sheet_IDs_Removed.xlsx*), the USV statistical data for the 6 month old animals (*NP_6mo_USV_IDs_Removed.xlsx*), the USV statistical data for the 10 month old animals (*NP_USV_10mo_IDs_Removed.xlsx*), the tongue force data for both the 6 and 10 month old animals (*NP6_10Tongue_IDs_Removed.xlsx*), and the protocol used for collecting the relative optical density (*Optical_Density_Procedure_1.28.2022.docx*).

**Abbreviations**

WT- Wild type

RoD- Relative Optical Density

12N- Hypoglossal Nucleus

Rob- Nucleus Raphe Obscurus

N/A- Not applicable, used to fill cells that were missing/did not have ...