The comparison of gene expression profiles in hepatic stellate cells isolated from mouse livers by perfusion method at 37 degrees or 6 degrees

We compared transcriptome profiling (bulk RNA-seq) of hepatic stellate cells isolated from mouse livers by perfusion method using collagenase at 37 degrees or 6 degrees and evaluated the heat-stressed gene expression at 37 degrees. Overall design: To scrutinize the side effects of high temperature incubation on the transcriptome, we examined the gene expression profoles in hepatic stellate cells isolated from mouse livers by perfusion method at 37 degrees or 6 degrees