COGs classification and bioinformatics analysis of R. heilongjiangensis surface-exposed proteins identified by ESI-MS/MS.

aSignal peptides and signal peptide types for all of the proteins were predicted with SignalP 3.0 or LipoP 1.0 software available online (http://www.cbs.dtu.dk/services/SignalP-3.0 and http://www.cbs.dtu.dk/services/LipoP-1.0. The websites were accessed on 22 January, 2013).bThe subcellular location of each protein was predicted with PSORTb 3.0.2 or SOSUI-GramN (http://www.psort.org/psortb/index.html and http://bp.nuap.nagoya-u.ac.jp/sosui/sosuigramn/sosuigramn_submit.html. The websites were accessed on 22 January, 2013).cProteins with homology to some R. heilongjiangensis surface-exposed proteins were also identified on the surfaces or in membrane extracts of some other rickettsiae. 1, 2, 3, 4, 5, and 6 refer to R. conorii[15], [36], [37], R. felis[38], R. parkeri[39], R. typhi[40], Anaplasma phagocytophilum[24], and Ehrlichia chaffeensis[25], respectively.dNon-classically secreted proteins were predicted with SecretomeP 2.0 (http://www.cbs.dtu.dk/services/SecretomeP/. The website was accessed on 22 January, 2013).SpI: signal peptide (signal peptidase I); EC: Extracellular; OM: Outer membrane; PP: Periplasmic; IM: Inner membrane. CYT: Cytoplasmic; Non-CYT: Non-Cytoplasmic; BCR: Bacterial conserved region.