PGC7-interacting proteins in HEK293T cells

Primordial germ cell 7 (PGC7), a maternal factor essential for early development, plays a critical role in the regulation of DNA methylation, transcriptional repression, chromatin condensation, and cell division and the maintenance of cell pluripotentiality. Despite the fundamental roles of PGC7 in these cellular processes, only a few molecular and functional interactions of PGC7 have been reported. Here, a streptavidin–biotin affinity purification technique combined with LC−MS/MS was used to analyze potential proteins that interact with PGC7. In total, 291 potential PGC7-interacting proteins were identified.Knowledge of these new PGC7-interacting proteins will likely explain the findings of previous studies on PGC7 and provide a substantial resource for subsequent in-depth studies on PGC7 functions.