Astrocyte-derived extracellular vesicles enhance the electrophysiological function and cell survival of human cortical neurons in vitro

The immunostained image analysis of neurons created, NTA analysis of obtained EVs, image-based demonstration of EV-uptake by neurons, FACS analysis to quantify apoptotic behavior of cultures of interest, beta-galactosidase result to explain cell senescence status in each group, patch clamp experiment result to demonstrate the electrophusiological effect of astroglial EV treatment.该数据集涵盖以下实验相关内容：所构建神经元的免疫染色图像分析，所获细胞外囊泡（Extracellular Vesicles, EVs）的纳米颗粒跟踪分析（Nanoparticle Tracking Analysis, NTA），基于成像手段展示神经元对细胞外囊泡的摄取过程，用于量化目标培养体系凋亡表型的荧光激活细胞分选术（Fluorescence-Activated Cell Sorting, FACS）分析，用以阐明各组细胞衰老状态的β-半乳糖苷酶（β-galactosidase）实验结果，以及用以展示星形胶质细胞来源细胞外囊泡处理所产生电生理效应的膜片钳实验结果。