Single-cell RNA gene expression profiles of the somites and myotomes at embryonic stages E18, E21, and E28 from Tibetan pigs (ZZ) and Duroc x Tibetan pigs (DZ)

To investigate the upstream regulatory networks in myogenesis that lead to the establishment of the myogenic lineage and subsequent differentiation, we proformed scRNA-seq of pig somite and myotome cells from Tibetan pigs (ZZ) and Duroc×Tibetan pigs (DZ) at several embryonic stages (E16, E18, E21, and E28). Overall design: Somite tissues [a mixture of embryos (n=5) from the same sow] of ZZ and DZ at E16, E18, E21, and myotome tissues [a mixture of embryos (n=5) from the same sow] at E28 were isolated, and the tissues were digested with collagenase for single-cell RNA sequencing.