WIN site inhibition disrupts a subset of WDR5 function

This study demonstrates the impact of WIN site inhibitors versus WDR5 degradation on H3K4me and transcriptional processes in human Burkitt's lymphoma cells. We use RNA-seq to measure global transcript levels, ChIP-seq to map genomic H3K4me3, and PRO-seq to map genomic polymerase density and primary transcripts. Our data show that WIN site inhibition disables only a specific subset of WDR5 activity, and that H3K4me changes induced by WDR5 depletion do not explain accompanying transcriptional responses. Overall design: Degrade WDR5 rapidly with Auxin (IAA) in AIDW Ramos cells and inhibit WDR5 WIN-site with C16 inhibitor.