RNA-Seq based transcriptome analysis of colorectal cancer cells in response to lactate treatment

Recently, the presence of lactate in colorectal cancer (CRC) has been rediscovered as crucial signalling molecule which plays key roles in the regulation of the metabolic pathways, the immune response, and cell-to-cell communication within the tumour microenvironment. We here report the RNA-Seq based transcriptome analysis of DLD1 and SW480 cells in response to lactate treatment. By a number of statistical tests, a series of genes were statistically significant, indicating a gene set profoundly responded to lactate in CRC. To unveil the molecular mechanism associated with the effects of lactate on CRC development and progress, these genes were annotated using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes functional enrichment analyses. Our data demonstrate that targeting lactate metabolism and transport represents a promising approach for CRC therapeutics. Examination of transcriptome in 2 CRC cell lines. For each cell line, 6 samples are analyzed RNA sequencing (3 biological replicates for control samples and 3 biological replicates for lactate treated samples).