Secretory MPP3 reinforce myeloid differentiation trajectory and amplify myeloid cell production

Hematopoietic stem cells (HSC) and downstream lineage-biased multipotent progenitors (MPP) tailor blood production and control myelopoiesis on demand. However, a precise resolution of myeloid differentiation trajectories and cellular heterogeneity in various MPP populations and understanding of their role in controlling myeloid cell production remains largely lacking. Here, we analyze that myeloid-biased MPP3 are functionally and molecularly heterogenous, with a distinct subset of myeloid-primed secretory cells with high endoplasmic reticulum (ER) volume. Overall design: Examination of 15 Bulk RNA seqs from hematopoietic stem and progenitor populations from mouse bone marrow, and 8 droplet-based single cell RNA seqs from MPP3 cells from mouse bone marrow