MaRNAV_Ethiopia_Code_Script

This deposit provides segment-resolved MaRNAV (Matryoshka RNA virus) consensus nucleotide sequences derived from a secondary analysis of publicly available paired-end RNA-seq runs (Kepple et al., 2024) from Plasmodium vivax samples from Ethiopia (Pv01–Pv03; SRR17069092, SRR17069099, SRR17069100). Reads were quality-trimmed and mapped to MaRNAV reference segment accessions (including MN698828.1 [RdRp segment] and MN698826.1 [hypothetical protein segment]), followed by variant/consensus calling to generate masked consensus FASTAs. Predicted coding regions were translated to produce protein ORFs (RdRp and “hypothetical protein/ORF2-like” products) used in downstream analyses. Sequence identity was validated using BLASTX and BLASTP, with raw BLAST reports and a compact TSV top-hit summary included for transparency. For phylogenetic context, the exact protein FASTA inputs, alignments, and maximum-likelihood tree outputs (including Newick tree files and IQ-TREE reports) are provided, along with manuscript-ready phylogram figures highlighting the Ethiopian sequences and their closest public relatives. All outputs are derived from de-identified public data; no new sampling was performed.