Detection of RABV in salivary gland and RABV shedding in saliva by LN34 RT-PCR in bats confirmed positive for rabies in brain tissue by the direct fluorescent antibody test.

According to a rotating group schedule, saliva samples were obtained on day of death (0) if possible, daily if clinical signs were observed, or 1–3 days before death. Symbols are: + “positive”,–“negative”, and “inc” inconclusive. Inconclusive results are those with a Ct value from 35–40 and could indicate low virus load, insufficient sample, or possible cross-contamination. A Ct value with n/d is “not detected”, n/a means sample was not available. Age groups are J = juvenile, A = adult, and p = pup. Clinical signs are categorized as furious (F) or paralytic (P), and time to death is the number of days from RABV challenge until the bat died or was euthanized.