Determination of fluoroquinolones concentration among biofilm-forming and non-forming uropathogenic Acinetobacter Calcoaceticus-Baumannii complex and their correlation to fluoroquinolone-resistant genes

Acinetobacter spp., the predominant cause of urinary tract infection (UTI), is increasingly resistant to fluoroquinolones. Resistance to fluoroquinolones poses a serious health problem in the treatment of urinary tract infections, which are mostly associated with chromosomal mutations encoded by topoisomerase IV. The objective of the study was to detect the blaOXA-23, qnrB, and acc(6l)-lb-cr genes in fluoroquinolone-resistant biofilm-producing ACB complex isolated from UTI-suspected patients.
A hospital-based cross-sectional study was carried out at the Nepal Police Hospital, Kathmandu, from April 2019 to January 2021. A total of 598 urine specimens were processed, and 78 ACB complex were isolated from a total of 366 positive cultures, which were identified based on colony morphology, Gram’s staining reaction, and various biochemical tests. The antimicrobial susceptibility pattern was determined by the modified Kirby-Bauer disk diffusion method, and the result was interpreted according ..., , , Determination of fluoroquinolones concentration among biofilm-forming and non-forming uropathogenic Acinetobacter Calcoaceticus-Baumannii complex and their correlation to fluoroquinolone-resistant genes

Dataset DOI link: https://doi.org/10.5061/dryad.2z34tmptj

Resistance to antimicrobials is rapidly emerging among uropathogens, chiefly attributed to the extensive use and misuse of antimicrobial agents. For example, fluoroquinolone, which was once considered a highly effective broad-spectrum antibiotic to treat infectious diseases, is currently of poor functionality because of the emergence of fluoroquinolone-resistant bacteria, limiting the selection for treating infections. Of the several resistance mechanisms to fluoroquinolones, plasmid-mediated quinolone resistance (PMQR) (blaoxa-23, aac(6')-Ib-cr, qnrB) and biofilm formation—the polymicrobial community of microbial cells enveloped by extracellular polymeric substances—have been identified as the major resistance mechanisms to flu...