RNA-seq analysis of the transcriptomic profiles of ATO+DAC-treated THP-1 cell lines expressing p53-R282W, vector control, or p53-R282W with IRF7 knockdown

To investigate the transcriptomic effects of ATO and DAC co-treatment in THP-1 cells expressing different p53 contexts, RNA-seq analysis was conducted on cell lines expressing p53-R282W, vector control, or p53-R282W with IRF7 knockdown. Cells were treated with arsenic trioxide (1 μg/mL) and decitabine (5 μM) prior to total RNA extraction. Purified mRNA was subjected to high-throughput sequencing using the Illumina HiSeq platform. Sequencing reads that passed quality control were trimmed using Cutadapt and used for downstream transcriptomic analysis. RNA-seq was performed to evaluate transcriptional activity of mutant p53 in response to the indicated treatments.