Role of HcpR in nitrosative stress protection. Role of HcpR in nitrosative stress protection

HcpR has been shown to be required for growth of Porphyromonas gingivalis in the presence of nitrite (Infect Immun. 2012 Sep;80(9):3319-31. doi: 10.1128/IAI.00561-12. Epub 2012 Jul 9). It also has been shown to regulate the expression of hcp. To define the entire regulon of HcpR RNAseq examination of the wild type and mutant (deficient in HcpR) strains have been used to define the regulon in response to nitrite exposure in bacteria grown in the presence and absence of nitrite. Four biological replicates were prepared for both, wild type and mutant strains grown in the presence and absence of nitrite. Overall design: Wild type Porphyromonas gingivalis W83 strain (2802) was used to compare to the hcpR-deficient strain (2807).