The importance of the bacterial cell wall in uranium (VI) biosorption

The bacterial cell envelope, in particular the cell wall, is considered
the main controlling factor in the biosorption of aqueous uranium (VI)
by microorganisms. However, the specific roles of the cell wall,
associated biomolecules, and other components of the cell envelope are
not well defined. Here we report findings on the biosorption of uranium
by isolated cell envelope components and associated biomolecules, with
P. putida 33015 and B. subtilis 168 investigated as representative
strains for the differences in Gram-negative and Gram-positive cell
envelope architecture, respectively. The cell wall and cell surface
membrane were isolated from intact cells using a French Pressure Cell
and characterised by X-Ray Photoelectron Spectroscopy (XPS) and
Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FT-IR)
spectroscopy. Uranium biosorption was investigated as a function of cell
envelope component and pH, comparing with intact cells and was
quantified by ICP-OES.Supplementary information includes characterisation of XPS spectra, uranium speciation, additional adsorption isotherms, langmuir fittings and detailed FT-IR characterisation of cell envelope components.Data includes raw XPS and FT-IR spectra, peak identifications and uranium isotherm fittings from data collected by ICP-OES.

细菌细胞包膜，尤其是细胞壁，被认为是微生物对水合铀（VI）生物吸附的主要控制因素。然而，细胞壁、相关生物大分子以及细胞包膜其他组成部分的特定作用尚不明确。本研究报告了通过分离细胞包膜组分和相关生物大分子对铀的生物吸附的研究成果，其中以P. putida 33015和B. subtilis 168作为分别代表革兰氏阴性菌和革兰氏阳性菌细胞包膜结构的典型菌株进行了研究。采用法国压力细胞从完整细胞中分离出细胞壁和细胞表面膜，并通过X射线光电子能谱（XPS）和衰减全反射傅里叶变换红外（ATR-FT-IR）光谱对其进行表征。研究了细胞包膜组分和pH值对铀生物吸附的影响，并与完整细胞进行了比较，采用电感耦合等离子体质谱法（ICP-OES）进行定量。补充信息包括XPS光谱表征、铀物种鉴定、额外的吸附等温线、Langmuir拟合以及细胞包膜组分详细的FT-IR表征。数据包括由ICP-OES收集的原始XPS和FT-IR光谱、峰识别和铀等温线拟合。