Acer japonicum Iso-Seq analysis

This project aims to estimate the full-length amino acid sequences of Acer japonicum protein with high confidence using the full-length cDNA sequences obtained by isoform sequencing (Iso-Seq) analysis. RNA was extracted from the leaves of an adult A. japonicum tree (height: approximately 10 m, age: 50 years) grown in the arboretum of the Hokkaido Research Center, Forestry and Forest Products Research Institute (43.0°N, 141.4°E; 180 m a.s.l.) at approximately 10:00 am on July 7, and September 7, 2021. Poly A+ RNA was further purified and analyzed using Sequel II (Pacific Biosciences). Raw data were processed using SMRT Link v6.0 (Pacific Biosciences) and then further processed using IsoSeq3 tools (version 3.1).