RNA sequencing of mouse samples using the Illumina TruSeq? Stranded mRNA technology and the NextSeq500 next generation sequencing system

RNA sequencing analysis was performed on mouse cell samples. Quality control of total RNA samples after RNA isolation with the Agilent Bioanalyzer revealed acceptable RNA quality for 12 out of 30 provided samples. Libraries were successfully prepared from these RNA samples using the Illumina TruSeq? Stranded mRNA technology. This approach uses fragmentation and sequencing adapter ligation in combination with a poly-T oligo pulldown. RNA sequencing was performed on the Illumina NextSeq500 next generation sequencing system (Illumina) and its high output mode with two runs of 1x75 bp single-end read chemistry and a high amount of high quality reads was generated.