bli-1 12 DAG continuous light grown whole seedlings analyzed on a 44k single color Agilent microarray

Polycomb group (PcG) proteins are key epigenetic regulators of development. The highly conserved Polycomb repressive complex 2 (PRC2) represses thousands of target genes by trimethylating H3K27 (H3K27me3). Plant specific PcG components and functions are largely unknown, however, we previously identified the plant-specific protein BLISTER (BLI) as a PRC2 interactor. BLI regulates PcG target genes and promotes cold stress resistance. To further understand the function of BLI, we analyzed the transcriptional profile of bli‑1 mutants. The goal of this experiment was to examine the transcriptional profile of bli-1 seedlings and compare it to the wild type Col-0. Each two biological replicates of Col-0 and bli-1 12 day old seedlings grown under continuous light conditions (grown on solid half-strength Murashige and Skoog medium supplemented with 0.5% sucrose and 0.05% MES) were compared here. Transcript levels of the two genotypes were evaluated using a 44k single colour Agilent microarray.