PFMCal: Photonic force microscopy calibration extended for its application in high-frequency microrheology

The present document is an update of the previously published MatLab code for the calibration of optical tweezers in the high-resolution detection of the Brownian motion of non-spherical probes. In this instance, an alternative version of the original code, based on the same physical theory, but focused on the automation of the calibration of measurements using spherical probes, is outlined. The new added code is useful for high-frequency microrheology studies, where the probe radius is known but the viscosity of the surrounding fluid maybe not. This extended calibration methodology is automatic, without the need of a user’s interface. A code for calibration by means of thermal noise analysis is also included; this is a method that can be applied when using viscoelastic fluids if the trap stiffness is previously estimated. The new code can be executed in MatLab and using GNU Octave. The previous version of this program (AEXH_v1_0) may be found at http://dx.doi.org/10.1016/j.cpc.2015.06.024本文件为已公开刊发的原MatLab代码的更新版本，该代码用于高分辨率检测非球形探针布朗运动时的光镊（optical tweezers）校准。本文在此概述了原代码的另一变体：其基于相同物理理论，专为球形探针的测量校准自动化流程设计。新增代码适用于高频微流变学研究：此类场景中探针半径已知，但周遭流体的粘度往往未知。该扩展校准方法实现全自动运行，无需用户交互界面。代码还集成了基于热噪声分析的校准模块：若此前已估算光阱刚度（trap stiffness），则该方法可用于粘弹性流体的相关实验。该新版代码可在MatLab及GNU Octave环境中运行。 该程序的旧版本（AEXH_v1_0）可通过以下链接获取：http://dx.doi.org/10.1016/j.cpc.2015.06.024