Crystal structure of GFP-LAMA-F98 - a GFP enhancer nanobody with cpDHFR insertion and TMP and NADPH

Crystal structure of GFP-LAMA-F98 - a GFP enhancer nanobody with cpDHFR insertion and TMP and NADPH Descriptor: GFP-LAMA-F98 a GFP enhancer nanobody with cpDHFR insertion,Dihydrofolate reductase,GFP-LAMA-F98 a GFP enhancer nanobody with cpDHFR insertion,Dihydrofolate reductase,Dihydrofolate reductase,GFP-LAMA-F98 a GFP enhancer nanobody with cpDHFR insertion,Dihydrofolate reductase,GFP-LAMA-F98 a GFP enhancer nanobody with cpDHFR insertion, NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, TRIETHYLENE GLYCOL, ... Authors: Farrants, H, Tarnawski, M, Mueller, T.G, Otsuka, S, Hiblot, J, Koch, B, Kueblbeck, M, Kraeusslich, H.-G, Ellenberg, J, Johnsson, K. Deposit date: 2019-05-28 Release date: 2020-02-12 Last modified: 2024-11-13 Method: X-RAY DIFFRACTION (2.2 Å) Cite: Chemogenetic Control of Nanobodies. Nat.Methods, 17, 2020

GFP-LAMA-F98的晶体结构——一种带有cpDHFR（循环型二氢叶酸还原酶）插入片段、可结合TMP（甲氧苄啶）与还原型烟酰胺腺嘌呤二核苷酸磷酸（NADPH）的GFP增强型纳米抗体。
描述：GFP-LAMA-F98为带有cpDHFR插入片段的GFP增强型纳米抗体，相关组分包含二氢叶酸还原酶；涉及的试剂包括还原型烟酰胺腺嘌呤二核苷酸磷酸（全称DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE，即NADPH）与三甘醇（TRIETHYLENE GLYCOL）等。
作者：Farrants H、Tarnawski M、Mueller T.G、Otsuka S、Hiblot J、Koch B、Kueblbeck M、Kraeusslich H.-G、Ellenberg J、Johnsson K。
存储日期：2019-05-28；发布日期：2020-02-12；最后修改日期：2024-11-13。
实验方法：X射线衍射（分辨率2.2 Å）。
引用文献：《纳米抗体的化学遗传调控》，《自然·方法》，第17卷，2020年。