Anomalous bromide substructure of NmHR under dark state conditions determined at 13.7 keV with serial crystallography

Anomalous bromide substructure of NmHR under dark state conditions determined at 13.7 keV with serial crystallography Descriptor: (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate, BROMIDE ION, Chloride pumping rhodopsin, ... Authors: Mous, S, Gotthard, G, Ehrenberg, D, Sen, S, James, D, Johnson, P, Weinert, T, Nass, K, Furrer, A, Kekilli, D, Ma, P, Bruenle, S, Casadei, C, Martiel, I, Dworkowski, F, Gashi, D, Skopintsev, P, Wranik, M, Knopp, G, Panepucci, E, Panneels, V, Cirelli, C, Ozerov, D, Schertler, G, Wang, M, Milne, C, Standfuss, J, Schapiro, I, Heberle, J, Nogly, P. Deposit date: 2021-04-15 Release date: 2022-02-09 Last modified: 2024-11-06 Method: X-RAY DIFFRACTION (1.75 Å) Cite: Dynamics and mechanism of a light-driven chloride pump. Science, 375, 2022

本数据集针对暗态条件下NmHR的异常溴化物亚结构开展解析，实验采用13.7 keV能量下的连续晶体学（serial crystallography）方法完成测定。数据集描述项包括：(2R)-2,3-二羟基丙基 (9Z)-十八碳-9-烯酸酯、溴离子（BROMIDE ION）、氯离子泵视紫红质（Chloride pumping rhodopsin）等。作者：Mous S、Gotthard G、Ehrenberg D、Sen S、James D、Johnson P、Weinert T、Nass K、Furrer A、Kekilli D、Ma P、Bruenle S、Casadei C、Martiel I、Dworkowski F、Gashi D、Skopintsev P、Wranik M、Knopp G、Panepucci E、Panneels V、Cirelli C、Ozerov D、Schertler G、Wang M、Milne C、Standfuss J、Schapiro I、Heberle J、Nogly P。数据沉积日期：2021-04-15；发布日期：2022-02-09；末次修改日期：2024-11-06。实验方法：X射线衍射（X-RAY DIFFRACTION），分辨率1.75 Å。引用文献：《光驱动氯离子泵的动力学与机制》（Dynamics and mechanism of a light-driven chloride pump），Science，第375卷，2022年。