3D ED tilt series of Pigment Red 5

Electron diffraction tilt series of Pigment Red 5. The experimental data were acquired using a 200 kV Glacios transmission electron microscope (Thermo Fisher) operating at liquid nitrogen temperature, employing the EPU-D (Thermo Fisher) module in continuous rotation mode. Electron diffraction patterns were collected in nanodiffraction mode, with an effective beam diameter of 1 µm on the sample. Tilt series were acquired within a goniometer tilt range of -60° to 70°, with a frame interval of 1° and a rotation speed of 0.5 s/1°. A binning factor of 4 was applied to the CETA camera, resulting in an effective pixel size of 0.002 Å^−1.