RNAseq anaysis of hippocamal cck cells of SMARCA3 cKO mice
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https://www.ncbi.nlm.nih.gov/sra/SRP234734
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Purpose: To identify gene expression changes in CCK neurons of hippocsmpus of SMARCA3 cKO mice Method: Translating Ribosome Affinity Purification (TRAP) to isolate RNA from CCK+ cells and, cDNA synthesis and next generation RNAseq using Illumina Nextseq sequencer. Results: Biostatistical analysis identified 1378 genes that were altered by SMARCA3 cKO. Overall design: 4 hippocampi were pooled for one translating ribosome affinity purification sample. We used mice that were CCK cre+ crossed with L10-EGFP+ mice and SMARCA3 fl/fl and littermates without SMARCA3 fl/fl controls.
创建时间：
2023-02-11
