Temperature-induced transcriptome profiles of endosprem and embryo during seed development and germination in Brassica oleracea

To understand how endosperm and embryo perceive temperature signals to control seed vigour in Brassica oleracea, we grow plants in 16°C till bent-cotyledon stage, which is a key stage sensing temperature. Then plants were either transferred to 26°C or kept in 16°C. Endosperm/seed coat and embryo RNA samples were collected for RNA-seq at day0, day1, day3, day7, day18 and day22. Further, RNA-seq was also performed in imbibed seeds matured in 16°C and 26°C, at 1 hour, 24 hours and 72 hour after imbibition. We investigated transcriptome profiles at 10 time points in Brassica endosperm/seed coat and embryo in response to different temperatures